Anti-Midkine antibody (ab36038)
Key features and details
- Rabbit polyclonal to Midkine
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Midkine antibody
See all Midkine primary antibodies -
Description
Rabbit polyclonal to Midkine -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Rabbit, Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Midkine.
Read Abcam's proprietary immunogen policy (Peptide available as ab36571.) -
Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab36038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 16 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. -
Sequence similarities
Belongs to the pleiotrophin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 280852 Cow
- Entrez Gene: 4192 Human
- Entrez Gene: 17242 Mouse
- Entrez Gene: 81517 Rat
- Omim: 162096 Human
- SwissProt: P21741 Human
- SwissProt: P12025 Mouse
- SwissProt: Q9R1S9 Rat
see all -
Alternative names
- Amphiregulin associated protein antibody
- Amphiregulin-associated protein antibody
- ARAP antibody
see all
Images
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Western blot - Anti-Midkine antibody (ab36038)All lanes : Anti-Midkine antibody (ab36038) at 1 µg/ml
Lane 1 : Recombinant Human Midkine protein at 0.1 µg
Lane 2 : DU145 Whole Cell Lysate at 10 µg
Lane 3 : SHSY5Y Whole Cell Lysate at 10 µg
Lane 4 : Mouse Ovary Tissue Lysate at 10 µg
Lane 5 : E14 Rat Brain Tissue Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16,42 kDa why is the actual band size different from the predicted?
Additional bands at: 76 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab36038 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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Western blot - Anti-Midkine antibody (ab36038)Anti-Midkine antibody (ab36038) at 1 µg/ml +Recombinant Human Midkine protein (ab49848) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Exposure time: 10 seconds -
Western blot - Anti-Midkine antibody (ab36038)Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Midkine protein with tag at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
The observed molecular weight of 42 kDa is the combined molecular weight of the full-length recombinant Midkine protein (~140 amino-acids) and the tag. -
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody (ab36038)ICC/IF image of ab36038 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36038, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (2)
ab36038 has been referenced in 2 publications.
- He D et al. Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain. J Neurochem 135:508-21 (2015). PubMed: 26206265
- Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). PubMed: 24039874
Images
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Western blot - Anti-Midkine antibody (ab36038)All lanes : Anti-Midkine antibody (ab36038) at 1 µg/ml
Lane 1 : Recombinant Human Midkine protein at 0.1 µg
Lane 2 : DU145 Whole Cell Lysate at 10 µg
Lane 3 : SHSY5Y Whole Cell Lysate at 10 µg
Lane 4 : Mouse Ovary Tissue Lysate at 10 µg
Lane 5 : E14 Rat Brain Tissue Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16,42 kDa why is the actual band size different from the predicted?
Additional bands at: 76 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab36038 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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Western blot - Anti-Midkine antibody (ab36038)Anti-Midkine antibody (ab36038) at 1 µg/ml +
Recombinant Human Midkine protein (ab49848) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Exposure time: 10 seconds -
Western blot - Anti-Midkine antibody (ab36038)Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Midkine protein with tag at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
The observed molecular weight of 42 kDa is the combined molecular weight of the full-length recombinant Midkine protein (~140 amino-acids) and the tag. -
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody (ab36038)ICC/IF image of ab36038 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36038, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
