Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human HNRNPK ELISA Kit (ab235652)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 84 pg/ml
  • Range: 117.19 pg/ml - 7500 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human HNRNPK ELISA Kit
    See all hnRNP K kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell extract 8 7.5%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    84 pg/ml

  • Range

    117.19 pg/ml - 7500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 120 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human HNRNPK ELISA Kit (ab235652) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HNRNPK protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HNRNPK with 84 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human HNRNPK Capture Antibody 1 x 600µl
    10X Human HNRNPK Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human HNRNPK Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.

  • Sequence similarities

    Contains 3 KH domains.

  • Post-translational
    modifications

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt

  • Alternative names

    • CSBP
    • dC stretch binding protein
    • FLJ41122
    • Heterogeneous nuclear ribonucleoprotein K
    • hnRNP K
    • HNRNPK
    • HNRPK
    • HNRPK_HUMAN
    • Transformation up regulated nuclear protein
    • Transformation up-regulated nuclear protein
    • Transformation upregulated nuclear protein
    • TUNP
    see all

  • Database links

    Images

    • Other - Human HNRNPK ELISA Kit (ab235652)
      Other - Human HNRNPK ELISA Kit (ab235652)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human HNRNPK standard curve in 1X Cell Extraction Buffer PTR.
      Example of human HNRNPK standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native HNRNPK in MCF-7 extract and Raw264.7 extract based on a 12.5 µg/mL and 12.5 µg/mL extract loads, respectively.
      Interpolated concentrations of native HNRNPK in MCF-7 extract and Raw264.7 extract based on a 12.5 µg/mL and 12.5 µg/mL extract loads, respectively.

      The concentrations of HNRNPK were measured in duplicate and interpolated from the HNRNPK standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HNRNPK concentration was determined to be 2,847 pg/mL in MCF-7 extract and 1,384 pg/mL in Raw264.7 extract.

    • Sandwich ELISA - Human HNRNPK ELISA Kit (ab235652)
      Sandwich ELISA - Human HNRNPK ELISA Kit (ab235652)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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