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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker

Human Glypican 1 Antibody Pair - BSA and Azide free (GPC1) (ab253314)

Human Glypican 1 Antibody Pair - BSA and Azide free (GPC1) (ab253314)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Glypican 1 Antibody Pair - BSA and Azide free (GPC1)
    See all Glypican 1/ GPC1 kits
  • Assay type

    ELISA set
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Glypican 1 (GPC1). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270217), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Glypican 1 Capture Antibody (unconjugated) 1 x 100µg
    Human Glypican 1 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • HSPGs
    • Cancer
    • Signal transduction
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
  • Function

    Cell surface proteoglycan that bears heparan sulfate.
  • Sequence similarities

    Belongs to the glypican family.
  • Post-translational
    modifications

    This cell-associated glypican is further processed to give rise to a medium-released species.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Target information above from: UniProt accession P35052 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FLJ38078
    • glypican
    • Glypican proteoglycan 1
    • glypican1
    • GPC 1
    • Gpc1
    • GPC1_HUMAN
    • Secreted glypican-1
    see all
  • Database links

    • Entrez Gene: 2817 Human
    • Omim: 600395 Human
    • SwissProt: P35052 Human
    • Unigene: 328232 Human

    Images

    • Sandwich ELISA - Human Glypican 1 Antibody Pair - BSA and Azide free (GPC1) (ab253314)
      Sandwich ELISA - Human Glypican 1 Antibody Pair - BSA and Azide free (GPC1) (ab253314)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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