Anti-Caspr antibody [EPR23439-48] (ab246501)
![Anti-Caspr antibody [EPR23439-48] (ab246501)](https://www.abcam.com/ps/products/246/ab246501/Images/ab246501-394054-anti-caspr-antibody-epr2343948-western-blot.jpg "Anti-Caspr antibody [EPR23439-48] (ab246501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23439-48] to Caspr
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Caspr antibody [EPR23439-48]
See all Caspr primary antibodies -
Description
Rabbit monoclonal [EPR23439-48] to Caspr -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hela, SK-N-BE, SH-SY5Y, U-87 MG, IMR32, Neuro 2a and PC-12 whole cell lysates. IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23439-48 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspr antibody [EPR23439-48] (ab246501)All lanes : Anti-Caspr antibody [EPR23439-48] (ab246501) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SK-N-BE (human neuroblastoma neuroblast), whole cell lysate
Lane 3 : H-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 5 : MR-32 (human neuroblastoma neuroblast), whole cell lysate
Lane 6 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 156 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times. Lanes: 1 & 7, 26 seconds; Lanes: 2-6, 114 seconds.
Samples are non-boiled as boiling may cause protein aggregates.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)](https://www.abcam.com/ps/products/246/ab246501/Images/ab246501-1-anti-caspr-antibody-epr2343948-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Caspr with ab246501 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human cerebrum (PMID: 27488240). The section was incubated with ab246501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)](https://www.abcam.com/ps/products/246/ab246501/Images/ab246501-2-anti-caspr-antibody-epr2343948-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Caspr with ab246501 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse cerebrum (PMID: 27488240). The section was incubated with ab246501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)](https://www.abcam.com/ps/products/246/ab246501/Images/ab246501-3-anti-caspr-antibody-epr2343948-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR23439-48] (ab246501)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Caspr with ab246501 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat cerebrum (PMID: 27488240). The section was incubated with ab246501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Anti-Caspr antibody [EPR23439-48] (ab246501)](https://www.abcam.com/ps/products/246/ab246501/Images/ab246501-5-benefits-of-recombinant-antibodies.png)
Anti-Caspr antibody [EPR23439-48] (ab246501)