Anti-MRF antibody - Oligodendrocyte Marker (ab85464)
Key features and details
- Rabbit polyclonal to MRF - Oligodendrocyte Marker
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MRF antibody - Oligodendrocyte Marker
See all MRF primary antibodies -
Description
Rabbit polyclonal to MRF - Oligodendrocyte Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human MRF aa 1100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0; Embryonic brain E14; Embryonic brain E16.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatRabbitApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).Target
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Function
Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. -
Tissue specificity
Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina. -
Sequence similarities
Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain. -
Domain
A transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator. -
Cellular localization
Membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 225908 Mouse
- SwissProt: Q3UR85 Mouse
- Unigene: 329716 Mouse
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Alternative names
- C11orf9 antibody
- Chromosome 11 open reading frame 9 antibody
- GM1804 antibody
see all
Images
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Western blot - Anti-MRF antibody - Oligodendrocyte Marker (ab85464)All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution
Lane 1 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab85464 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MRF antibody - Oligodendrocyte Marker (ab85464)All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution
Lane 1 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
