Human Glypican 1 ELISA Kit (GPC1) (ab270217)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16.16 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Glypican 1 ELISA Kit (GPC1)
See all Glypican 1/ GPC1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.8% Inter-assay Sample n Mean SD CV% Serum 3 5.2% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Cell Lysate, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16.16 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 110% - 117% Serum 112 106% - 117% Cell culture extracts 87 83% - 100% Tissue Extracts 97 87% - 111% Hep Plasma 99 96% - 101% EDTA Plasma 106 90% - 119% Cit plasma 87 81% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Glypican 1 ELISA Kit (GPC1) (ab270217) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glypican 1 (GPC1) protein in cell lysate, edta plasma, serum, urine, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glypican 1 (GPC1) with 16.16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Glypican 1 (GPC1) is synthesized as a 558 amino acid (aa) precursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment. The standard protein in this product is the mature form. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. GPC1 may play a role in the control of cell division and growth regulation. Human GPC1 shares 89%, 89%, and 87% sequence homology with mouse, rat, and bovine, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Glypican 1 (GPC1) Capture Antibody 1 x 600µl 10X Human Glypican 1 (GPC1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Glypican 1 (GPC1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Sequence similarities
Belongs to the glypican family. -
Post-translational
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- FLJ38078
- glypican
- Glypican proteoglycan 1
see all -
Database links
- Entrez Gene: 2817 Human
- Omim: 600395 Human
- SwissProt: P35052 Human
- Unigene: 328232 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Glypican 1 standard curve in Sample Diluent NS.The Glypican 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Glypican 1 in human serum and plasma samples.The concentrations of Glypican 1 were measured in duplicates, interpolated from the Glypican 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 11.98 ng/mL in serum, 9.90 ng/mL in plasma (citrate) and 10.44 ng/mL in plasma (EDTA), and 9.93 ng/mL in plasma (Heparin).
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Interpolated concentrations of native Glypican 1 in human cell culture supernatant, and urine samples.The concentrations of Glypican 1 were measured in duplicates, interpolated from the Glypican 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: MCF7 supernatant 25%, HepG2 supernatant 50%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 10.14 ng/mL in MCF7 supernatant, 4.5 ng/mL in HepG2 supernatant and 7.87 ng/mL in urine.
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Interpolated concentrations of native Glypican 1 in human MCF7 extract based on a 500 µg/mL and placenta extract based on a 1,000 µg/mL extract load.The concentrations of Glypican 1 were measured in duplicate and interpolated from the Glypican 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 4.48 ng/mL in MCF7 extract and 1.08 ng/mL in placenta.
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25% serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 12.53 ng/mL with a range of 9.90 – 17.46 ng/mL.
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Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (ab270217)To learn more about the advantages of recombinant antibodies see here.
