Human Flt3 ligand / Flt3L ELISA Kit (ab213780)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Flt3 ligand / Flt3L ELISA Kit
See all Flt3 ligand/Flt3L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 109pg/ml 3.49 3.2% 2 16 256pg/ml 8.45 3.3% 3 16 520pg/ml 22.88 4.4% Inter-assay Sample n Mean SD CV% 1 24 112pg/ml 10.64 9.5% 2 24 245pg/ml 18.38 7.5% 3 24 518pg/ml 41.96 8.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Flt3 ligand / Flt3L Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213780) is designed for the quantitative detection of Human Flt3 ligand / Flt3L in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Flt3 ligand / Flt3L has been pre-coated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt3 ligand / Flt3L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Flt3 ligand / Flt3L amount of sample captured in plate.
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Notes
Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt3 ligand / Flt3L or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt3 ligand / Flt3L is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand / Flt3L stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt3 ligand / Flt3L can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt3 ligand / Flt3L also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Flt3 ligand coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Flt3 ligand antibody 1 x 130µl Lyophilized recombinant Human Flt3 ligand standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- FL
- Flt 3 ligand
- Flt 3L
see all -
Database links
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
Images
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Human Flt3 ligand / Flt3L ELISA Kit (ab213780) Standard Curve
Human Flt3 ligand / Flt3L ELISA Kit (ab213780) Standard Curve