Human FABP5 Antibody Pair - BSA and Azide free (ab253361)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human FABP5 Antibody Pair - BSA and Azide free
See all FABP5 kits -
Assay type
ELISA set -
Range
0.313 ng/ml - 20 ng/ml -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human FABP5. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272474), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human FABP5 Capture Antibody (unconjugated) 1 x 100µg Human FABP5 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. -
Tissue specificity
Keratinocytes; highly expressed in psoriatic skin. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- CFABP
- Cutaneous fatty acid binding protein
- DA11
see all -
Database links
- Entrez Gene: 2171 Human
- Omim: 605168 Human
- SwissProt: Q01469 Human
- Unigene: 408061 Human
Images
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Sandwich ELISA - Human FABP5 Antibody Pair - BSA and Azide free (ab253361)To learn more about the advantages of recombinant antibodies see here.
