Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human FABP5 ELISA Kit (ab272474)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.14 ng/ml
  • Range: 0.31 ng/ml - 20 ng/ml
  • Sample type: EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human FABP5 ELISA Kit
    See all FABP5 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6%

  • Sample type

    Saliva, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.14 ng/ml

  • Range

    0.31 ng/ml - 20 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 99 96% - 102%
    Urine 83 81% - 83%
    Serum 105 94% - 116%
    Hep Plasma 93 81% - 101%
    EDTA Plasma 83 81% - 84%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human FABP5 ELISA kit (ab272474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human FABP5 protein in serum, plasma, urine, saliva and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP5 with 0.14 ng/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Fatty acid-binding protein 5 (FABP5), also known as E-FABP for Epidermal-type fatty acid-binding protein, is a member of the fatty acid binding proteins and is encoded by the FABP5 gene. FABP5 is an a 134- amino acid containing protein that can be found either as a monomer or a homodimer. FABP5 mainly serves as an intracellular carrier for long-chain fatty acids and related lipids, which regulates metabolism. FABP5 can also transport specific fatty acids, such as retinoic acid and endocannabinoid, from the cytosol to the nucleus, where they activate nuclear receptors.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human FABP5 standard curve in Sample Diluent NS.
    Example of human FABP5 standard curve in Sample Diluent NS.

    The FABP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native FABP5 in human saliva.
    Interpolated concentrations of native FABP5 in human saliva.

    The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 766 ng/mL in saliva.

  • Interpolated concentrations of native FABP5 in human urine.
    Interpolated concentrations of native FABP5 in human urine.

    The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 8.2 ng/mL in urine.

  • Serum from eight individual healthy FABP5 male donors was measured in duplicate.
    Serum from eight individual healthy FABP5 male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP5 concentration was determined to be 3.4 ng/mL with a range of 0.8 – 11.2 ng/mL.

  • Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
    Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)
    To learn more about the advantages of recombinant antibodies see here.

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