Human Endostatin ELISA Kit (ab100508)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 13.7 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Detection Antibody Endostatin 2 units Endostatin Microplate (12 strips x 8 wells) 1 unit Endostatin Standard Human(recombinant) (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. -
Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1)
- Alpha 1 type XVIII collagen
- Antiangiogenic agent
see all -
Database links
- Entrez Gene: 80781 Human
- Omim: 120328 Human
- SwissProt: P39060 Human
- Unigene: 517356 Human
Images
-
Typical Standard Curve
Representative Standard Curve using ab100508
-
Typical Standard CurveRepresentative Standard Curve using ab100508
