Human Endostatin ELISA Kit (ab184864)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.7 pg/ml
- Range: 32 pg/ml - 2000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Endostatin ELISA Kit
See all Endostatin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 6.9% Inter-assay Sample n Mean SD CV% Serum 3 7.1% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.7 pg/ml -
Range
32 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 99% - 114% Plasma 105 104% - 107% Cell culture media 88 87% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Endostatin ELISA kit (ab184864) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endostatin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Endostatin with 7.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Endostatin Capture Antibody 1 x 600µl 10X Human Endostatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Endostatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. -
Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1)
- Alpha 1 type XVIII collagen
- Antiangiogenic agent
see all -
Database links
- Entrez Gene: 80781 Human
- Omim: 120328 Human
- SwissProt: P39060 Human
- Unigene: 517356 Human
Images
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Other - Human Endostatin ELISA Kit (ab184864)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Figure 1Example of Endostatin standard curve. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Figure 2aTitration of normal Human serum and plasma samples to within working range of the assay. Background subtracted data from duplicate measurements are plotted.
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Figure 2bTitration of normal Human serum and plasma samples to within working range of the assay. Bar graph denotes quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.
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Figure 3Titration of individual donor serum samples to within working range of the assay. Quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.
