Human PD-1 ELISA Kit (ab252360)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.6 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PD-1 ELISA Kit
See all PD1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.4% Inter-assay Sample n Mean SD CV% Serum 3 9.6% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9.6 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 90% - 96% Tissue Extracts 102 101% - 104% Cell Lysate 98 95% - 100% Cell culture media 95 93% - 97% Hep Plasma 92 90% - 94% EDTA Plasma 95 93% - 97% Cit plasma 94 93% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human PD-1 ELISA Kit (ab252360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1 protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-1 with 9.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PD-1 Capture Antibody 1 x 600µl 10X Human PD-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PD-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Database links
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
Images
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Other - Human PD-1 ELISA Kit (ab252360)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PD-1 standard curve in Sample Diluent NS.The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human PD-1 standard curve in 1X Cell Extraction Buffer PTR.The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD-1 in human serum, plasma samples.The concentrations of PD-1 were measured in duplicates, interpolated from the PD-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (EDTA) neat, plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 119.1 pg/mL in serum, 88.1 pg/mL in plasma (citrate) and 85.9 pg/mL in plasma (EDTA), and 92.8 pg/mL in plasma (heparin).
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Interpolated concentrations of native PD-1 in human Molt-4 cell extract based on a 1000 ug/mL extract load and Thymus tissue extract samples based on a 500 µg/mL extract load.The concentrations of PD-1 were measured in duplicate and interpolated from the PD-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 636.9 pg/mL in Molt-4 cell extract and 566.5 pg/mL in Thymus tissue extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 86.56 pg/mL with a range of 47.82 – 210.99 pg/mL.
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Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)To learn more about the advantages of recombinant antibodies see here.
