Anti-HCC1 antibody (ab25801)
Key features and details
- Rabbit polyclonal to HCC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HCC1 antibody
See all HCC1 primary antibodies -
Description
Rabbit polyclonal to HCC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HCC1 aa 325-375. The exact sequence is proprietary.
Database link: Q14498 -
Epitope
The epitope recognized by this antibody maps to a region between residues 325 and 375 of human HCC1. -
Positive control
- IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HCC1 in HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HCC1 antibody (ab25801)All lanes : Anti-HCC1 antibody (ab25801) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 60 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (ab25801)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HCC1 with ab25801 at a 1/5000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-HCC1 antibody (ab25801)HCC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, with ab25801 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab25801 at 1 µg/ml.
Lane 1: ab25801 used for IP at 6 µg per reaction (IP by a previous lot of this antibody)
Lane 2: ab25801 used for IP at 6 µg per reaction
Lane 3: Control IgG.
Chemiluminescence detection: 30 seconds.
