Anti-LARP7 antibody (ab134757)
Key features and details
- Rabbit polyclonal to LARP7
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LARP7 antibody
See all LARP7 primary antibodies -
Description
Rabbit polyclonal to LARP7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide, corresponding to a region within the C terminal amino acids 532-582 of Human LARP7 (NP_056269.1).
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Positive control
- Whole cell lysate from HeLa, 293T, Jurkat and mouse NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LARP7 antibody (ab134757)All lanes : Anti-LARP7 antibody (ab134757) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from 293T cells at 50 µg
Lane 4 : Whole cell
lysate from Jurkat cells at 50 µg
Lane 5 : Whole cell
lysate from mouse NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds
Gel concentration 4-20% -
Immunoprecipitation - Anti-LARP7 antibody (ab134757)Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of LARP7.
Lane 2: Detection of Human LARP7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134757 at 6 µg/mg lysate for IP.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134757 at 1 µg/ml.
Gel concentration 4-20%
Developed using the ECL technique with an exposure time of 10 seconds.
