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Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)

Overview

  • Product name

    Human CCND2 (Cyclin D2) knockout HEK293T cell pellet
    See all Cyclin D2 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 8 bp insertion in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.

  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt

  • Alternative names

    • CCND 2
    • ccnd2
    • CCND2_HUMAN
    • CyclinD2
    • G1/S specific cyclin D2
    • G1/S-specific cyclin-D2
    • KIAK0002
    • MGC102758
    • MPPH3
    see all

Properties

Images

  • Western blot - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)
    Western blot - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)

    Lane 1: Wild-type HEK293T cell lysate (20 µg)
    Lane 2: CCND2 knockout HEK293T cell lysate (20 µg)

    Lanes 1-2: Merged signal (red and green). Green - ab207604 observed at 36 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab207604 Anti-Cyclin D2 antibody [EPR19659] was shown to specifically react with Cyclin D2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267318 (knockout cell lysate ab257875) was used.  Wild-type and Cyclin D2 knockout samples were subjected to SDS-PAGE.  ab207604 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.   Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)
    Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)

    Allele-1: 2 bp deletion in exon1

     

  • Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)
    Sanger Sequencing - Human CCND2 (Cyclin D2) knockout HEK293T cell pellet (ab278847)

    Allele-2: 8 bp insertion in exon1

     

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