Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12881] to Histone H2B (testis specific)
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-Histone H2B (testis specific) antibody [EPR12881]
    See all Histone H2B (testis specific) primary antibodies

  • Description

    Rabbit monoclonal [EPR12881] to Histone H2B (testis specific)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Histone H2B (testis specific) aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96A08

  • Positive control

    • Human and mouse testis lysates; Human testis tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12881

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    Western blot - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    All lanes : Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426) at 1/20000 dilution

    Lane 1 : Histone H2B1A (Q96A08) recombinant protein fragment (GST-Tag): aa2-101
    Lane 2 : Histone H2B1B (P33778) recombinant protein fragment (GST-Tag): aa2-126

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • Western blot - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    Western blot - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    All lanes : Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Mouse testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Histone H2B (testis specific) with ab178426 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    Immunoprecipitation - Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)

    Western blot analysis on immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab178426 at 1/10 dilution.

  • Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)
    Anti-Histone H2B (testis specific) antibody [EPR12881] (ab178426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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