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Cancer Cell cycle Cyclins

Anti-Cyclin A2 antibody (ab137769)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Cyclin A2 antibody (ab137769)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cyclin A2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cyclin A2 antibody
    See all Cyclin A2 primary antibodies
  • Description

    Rabbit polyclonal to Cyclin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment corresponding to Human Cyclin A2 (internal sequence). Recombinant fragment encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
    Database link: P20248

  • Positive control

    • 293T; A431; H1299; HeLaS3; HepG2; MOLT4; Raji and PC3 whole cell lysates; paraformaldehyde-fixed HeLa cells and paraffin-embedded NCIN87 xenograft.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin A Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin A Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin A family
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (ab137769)

    ab137769 detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by ab137769 diluted at 1/200.
    Red: phalloidin, a cytoskeleton marker diluted at 1/200.
    Blue: Hoechst 33342 staining.

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

    Lane 1 : SK-N-SH whole cell extract
    Lane 2 : IMR32 whole cell extract
    Lane 3 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Western blot - Anti-Cyclin A2 antibody (ab137769)
    Western blot - Anti-Cyclin A2 antibody (ab137769)
    Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (ab137769)
    Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution.
  • Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)
    Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)

    ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE: The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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