Anti-Cyclin A2 antibody (ab137769)
Key features and details
- Rabbit polyclonal to Cyclin A2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin A2 antibody
See all Cyclin A2 primary antibodies -
Description
Rabbit polyclonal to Cyclin A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to Human Cyclin A2 (internal sequence). Recombinant fragment encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.
Database link: P20248 -
Positive control
- 293T; A431; H1299; HeLaS3; HepG2; MOLT4; Raji and PC3 whole cell lysates; paraformaldehyde-fixed HeLa cells and paraffin-embedded NCIN87 xenograft.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab137769 detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by ab137769 diluted at 1/200.
Red: phalloidin, a cytoskeleton marker diluted at 1/200.
Blue: Hoechst 33342 staining. -
All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
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All lanes : Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution
Lane 1 : SK-N-SH whole cell extract
Lane 2 : IMR32 whole cell extract
Lane 3 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
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Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg
Predicted band size: 49 kDa
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Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 49 kDa
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Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution.
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ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE: The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.