HRP Anti-Tenascin C antibody [EPR4219] (ab196918)
![HRP Anti-Tenascin C antibody [EPR4219] (ab196918)](https://www.abcam.com/ps/products/196/ab196918/Images/ab196918-241950-WBab1969181.jpg "HRP Anti-Tenascin C antibody [EPR4219] (ab196918)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4219] to Tenascin C
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Tenascin C antibody [EPR4219]
See all Tenascin C primary antibodies -
Description
HRP Rabbit monoclonal [EPR4219] to Tenascin C -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain tissue lysate. IHC-P: Embryonic mouse brain (e17).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4219 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Tenascin C antibody [EPR4219] (ab196918)HRP Anti-Tenascin C antibody [EPR4219] (ab196918) at 1/5000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 241 kDa
Observed band size: 230,270 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196918 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Tenascin C antibody [EPR4219] (ab196918)](https://www.abcam.com/ps/products/196/ab196918/Images/ab196918-5-benefits-of-recombinant-antibodies.png)
HRP Anti-Tenascin C antibody [EPR4219] (ab196918)
