Anti-Tenascin C antibody [T2H5] (ab3970)
![Anti-Tenascin C antibody [T2H5] (ab3970)](https://www.abcam.com/ps/products/3/ab3970/Images/ab3970_1.jpg "Anti-Tenascin C antibody [T2H5] (ab3970)")
Key features and details
- Mouse monoclonal [T2H5] to Tenascin C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Tenascin C antibody [T2H5]
See all Tenascin C primary antibodies -
Description
Mouse monoclonal [T2H5] to Tenascin C -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).
-
Positive control
- IHC-P: Human tonsil tissue. WB: U-2 OS cell lysate.
-
General notes
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody notes
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. -
Clonality
Monoclonal -
Clone number
T2H5 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (ab3970)
ab3970 staining paraffin embedded tonsil tissue sections.
-
![Western blot - Anti-Tenascin C antibody [T2H5] (ab3970)](https://www.abcam.com/ps/products/3/ab3970/Images/ab3970-8441-anti-tenascin-c-antibody-t2h5-western-blot.jpg)
Western blot - Anti-Tenascin C antibody [T2H5] (ab3970)Anti-Tenascin C antibody [T2H5] (ab3970) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 265 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Tenascin C contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
