HRP Anti-Phosphothreonine-X-Phosphotyrosine antibody (ab9342)
Key features and details
- HRP Rabbit polyclonal to Phosphothreonine-X-Phosphotyrosine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Phosphothreonine-X-Phosphotyrosine antibody -
Description
HRP Rabbit polyclonal to Phosphothreonine-X-Phosphotyrosine -
Host species
Rabbit -
Conjugation
HRP -
Specificity
ELISA test indicated that this antibody specifically reacted to peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacted to pT-P-pY sequence. The antibody does not react with phosphothreonine and phosphoserine but reacts weakly with phosphotyrosine. -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugate.
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Positive control
- Mouse brain extract for immunoblotting. Synthetic pT-X-pY containing phosphopeptide for ELISA.
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General notes
Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with N-acetyl-phosphothreonine-lysine-phosphotyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose. -
Primary antibody notes
Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Phosphothreonine-X-Phosphotyrosine antibody (ab9342)
Immunoblotting of a human fibroblast cell lysate (4 hour UV treated and non-treated) with anti-pT-X-pY antibodies.
Immunoblotting of a human fibroblast cell lysate (4 hour UV treated and non-treated) with anti-pT-X-pY antibodies. -
Western blot - HRP Anti-Phosphothreonine-X-Phosphotyrosine antibody (ab9342)Immunoblotting of protein extracts with anti-pT-X-pY antibodies
A: Extract from Mouse Brain
B: Extract from Mouse Brain teated with Okadaic Acid(0.1 uM, 30 min).
C: Lysate of Melanoma Cell
D: Lysate of Melanoma Cell treated with Okadaic Acid.Immunoblotting of protein extracts with anti-pT-X-pY antibodies
A: Extract from Mouse Brain
B: Extract from Mouse Brain teated with Okadaic Acid(0.1 uM, 30 min).
C: Lysate of Melanoma Cell
D: Lysate of Melanoma Cell treated with Okadaic Acid.
