Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
Key features and details
- Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody -
Description
Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline -
Host species
Rabbit -
Specificity
This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule: TpP conjugated to KLH.
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Positive control
- WB: Phosphorylated MBP
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General notes
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose. -
Primary antibody notes
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)All lanes : Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
Lane 1 : Phosphorprotein protein kinase A PKA
Lane 2 : Myelin Basic Protein (MBP)