Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein (ab82118)

Key features and details

  • Expression system: Baculovirus
  • Purity: > 95% Affinity purified
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein

  • Purity

    > 95 % Affinity purified.
    ab82118 is greater than 95% homogeneous based on SDS-PAGE analysis, purified by affinity and FPLC chromatography.

  • Endotoxin level

  • Expression system

    Baculovirus

  • Accession

    P03070

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Simian Virus 40

    • Sequence

      MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEE KMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNE ENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAV FSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTP HRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPG GLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFE MCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRV DSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPK MDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNV NLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDY LDGSVKVNLEKKHLNKRTQIFPPGIVTMNEFSVPKTLQARFVKQIDFRAK DYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWK ERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNED GGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKK PPTPPPEPET

    • Amino acids

      1 to 708

    • Tags

      His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab82118 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.9
      Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • Large T antigen
      • LT
      • LTAG
      • Simian virus 40

    • Relevance

      SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

    • Cellular localization

      Host nucleus

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab82118? Please let us know so that we can cite the reference in this datasheet.

ab82118 has been referenced in 1 publication.

  • Jiang N  et al. Ultrasound-targeted microbubbles combined with a peptide nucleic acid binding nuclear localization signal mediate transfection of exogenous genes by improving cytoplasmic and nuclear import. Mol Med Rep 16:8819-8825 (2017). PubMed: 28990051

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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