HRP Anti-p53 antibody [DO-1] (ab204452)
![HRP Anti-p53 antibody [DO-1] (ab204452)](https://www.abcam.com/ps/products/204/ab204452/Images/ab204452-312598-anti-p53-antibody-do-1-hrp-western-blot.jpg "HRP Anti-p53 antibody [DO-1] (ab204452)")
Key features and details
- HRP Mouse monoclonal [DO-1] to p53
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-p53 antibody [DO-1]
See all p53 primary antibodies -
Description
HRP Mouse monoclonal [DO-1] to p53 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human p53 (N terminal). Epitope is within aa 20-25.
Database link: P04637 -
Epitope
ab204452 recognizes an epitope within aa 20-25 at the N-terminus of Human p53. -
Positive control
- WB: HEK293, DU 145 and HepG2 + Etopside whole cell lysate.
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General notes
Alternative versions available:
Anti-p53 antibody [DO-1] (ab1101) - Knockout validated
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
DO-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-p53 antibody [DO-1] (ab204452)All lanes : HRP Anti-p53 antibody [DO-1] (ab204452) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TP53 (p53) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa
Exposure time: 30 secondsab204452 was shown to specifically react with p53 in wild-type HAP1 cells as signal was lost in TP53 (p53) knockout cells. Wild-type and TP53 (p53) knockout samples were subjected to SDS-PAGE. Ab204452 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-p53 antibody [DO-1] (ab204452)](https://www.abcam.com/ps/products/204/ab204452/Images/ab204452-252320-anti-p53-antibody-do-1-hrp-western-blot.jpg)
Western blot - HRP Anti-p53 antibody [DO-1] (ab204452)All lanes : HRP Anti-p53 antibody [DO-1] (ab204452) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate + Etopside
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204452 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
