Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517)

Key features and details

  • HRP Mouse monoclonal [mAbcam 31830] to Histone H4
  • Suitable for: WB, IHC-P
  • Reacts with: Cow, Human, Recombinant fragment
  • Conjugation: HRP
  • Isotype: IgG1

Overview

  • Product name

    HRP Anti-Histone H4 antibody [mAbcam 31830]
    See all Histone H4 primary antibodies

  • Description

    HRP Mouse monoclonal [mAbcam 31830] to Histone H4

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Cow, Human, Recombinant fragment
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab13843)

  • Positive control

    • WB: Calf thymus histone preparation nuclear lysate. HeLa whole cell lysate (ab150035). Histone H4 recombinant protein. IHC-P: Normal human colon tissue.

  • General notes

     

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    mAbcam 31830

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

  • Light chain type

    kappa

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517)

    IHC image of Histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197517 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517)
    Western blot - HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517)
    All lanes : HRP Anti-Histone H4 antibody [mAbcam 31830] (ab197517) at 1/5000 dilution

    Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
    Lane 2 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 3 : Histone H4 Recombinant Protein at 0.1 µg
    Lane 4 : Histone H3.1 Recombinant Protein at 0.1 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197517 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

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