Anti-Ago1 antibody (ab5070)
Key features and details
- Rabbit polyclonal to Ago1
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Ago1 antibody
See all Ago1 primary antibodies -
Description
Rabbit polyclonal to Ago1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab23538)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Drosophila melanogasterAll applications MouseApplication Abreviews Notes WB (3) Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.Notes WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 7398 Drosophila melanogaster
- Unigene: 30800 Mouse
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Alternative names
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
Images
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (38)
ab5070 has been referenced in 38 publications.
- Adiliaghdam F et al. A Requirement for Argonaute 4 in Mammalian Antiviral Defense. Cell Rep 30:1690-1701.e4 (2020). PubMed: 32049003
- Goh E & Okamura K Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes. Nucleic Acids Res 47:3101-3116 (2019). PubMed: 30590701
- Wessels HH et al. Global identification of functional microRNA-mRNA interactions in Drosophila. Nat Commun 10:1626 (2019). PubMed: 30967537
- Mondal T et al. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS One 13:e0190548 (2018). PubMed: 29385168
- Zhou L et al. Importance of miRNA stability and alternative primary miRNA isoforms in gene regulation during Drosophila development. Elife 7:N/A (2018). PubMed: 30024380
Images
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
-
Western blot - Anti-Ago1 antibody (ab5070) This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
