Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
Key features and details
- Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed
- Conjugation: IRDye® 800CW
- Host species: Goat
- Isotype: IgG
- Suitable for: In-Cell ELISA, WB
Overview
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Product name
Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed
See all IgG secondary antibodies -
Description
Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: In-Cell ELISA, WBmore details -
Minimal
cross-reactivity
Goat, Horse, Human, Rabbit, Rat more details -
Immunogen
Full length protein corresponding to Mouse IgG.
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Conjugation
IRDye® 800CW
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using pooled mouse IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)All lanes : Anti-p53 antibody [DO-1] - ChIP Grade (ab1101) at 1/1000 dilution (Anti-p53 antibody [DO-1])
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : p53 knockout HAP1 cell lysate (20 µg)
Lane 3 : A431 cell lysate (20 µg)
Lane 4 : Saos-2 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab1101 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Image shows merged signal (red and green). Green - ab1101 observed at 53 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777
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Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution (Anti-beta Actin antibody [AC-15])
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab6276 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Image shows merged signal (red and green). Green - ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777
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In-Cell ELISA - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 for detection among its components. -
Spectrum - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
