Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448)
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]
See all NEDD4-2 primary antibodies -
Description
Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448.
Database link: Q96PU5 -
Positive control
- 293T cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8269(2) -
Isotype
IgG -
Research areas
Images
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Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa
Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands.
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