This kit allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent triglyceride substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258.

Lipases are a family of enzymes that release fatty acids from triacylglycerols in a site specific manner. Most lipases have optimum activity for the primary ester groups of triglycerides, while some lipases remove fatty acyl groups from either the C-1 or C-3 acyl positions. The substrate is typically not a single molecule, but a nonaqueous phase of aggregated lipid. Lipase activity, ubiquitous among most cells, can be monitored using the fluorescent substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol contained in the kit. Upon cleavage, the fluorescent fatty acid pyrenedecanoic acid is released and activity measurements are easily obtained either in vitro, in cell preparations, or in vivo.

This kit is sold by Marker Gene under product code M0612.