Anti-GPR91 antibody (ab41505)
Key features and details
- Rabbit polyclonal to GPR91
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GPR91 antibody
See all GPR91 primary antibodies -
Description
Rabbit polyclonal to GPR91 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Human GPR91 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab75121) -
Positive control
- This antibody gave a positive signal in both Human and Rat kidney tissue lysates as well as the following whole cell lysates: HEK293; A498; HUVEC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatApplication Abreviews Notes WB 1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Notes WB
1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Target
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Function
Receptor for succinate. -
Tissue specificity
Expressed specifically in kidney. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 56670 Human
- Entrez Gene: 408199 Rat
- Omim: 606381 Human
- SwissProt: Q9BXA5 Human
- SwissProt: Q6IYF9 Rat
- Unigene: 279575 Human
- Unigene: 124512 Rat
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Alternative names
- G protein coupled receptor 91 antibody
- G-protein coupled receptor 91 antibody
- GPR 91 antibody
see all
Images
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Western blot - Anti-GPR91 antibody (ab41505)All lanes : Anti-GPR91 antibody (ab41505) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Rat) Tissue Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 100 kDa, 49 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (1)
ab41505 has been referenced in 1 publication.
- Gao Y et al. Superparamagnetic iron oxide nanoparticle-mediated expression of miR-326 inhibits human endometrial carcinoma stem cell growth. Int J Nanomedicine 14:2719-2731 (2019). PubMed: 31114192
Images
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Western blot - Anti-GPR91 antibody (ab41505)All lanes : Anti-GPR91 antibody (ab41505) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Rat) Tissue Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 100 kDa, 49 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
