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FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)

Key features and details

  • FITC Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-EpCAM antibody [B29.1 (VU-1D9)]
    See all EpCAM primary antibodies

  • Description

    FITC Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    This antibody reacts with a 40 kD cell surface glycoprotein called ESA.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human EpCAM. Raised against carcinoma cell line of human origin.
    Database link: P16422

  • Positive control

    • Breast, colon carcinoma and tonsil

  • General notes

    Dilute according to the particular application being used. In general, 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

    Fluorescein (FITC) conjugated at a ratio of 6 moles of Fluorescein to 1 mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm and maximum emission occurs at wavelength 520 nm.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-EpCAM antibody [B29.1 (VU-1D9)] (ab8666)
    ICC/IF image of ab8666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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