Caspase-6 Inhibitor Assay Kit (ab102494)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Caspase-6 Inhibitor Assay Kit
See all Caspase-6 kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay time
1h 30m -
Product overview
Abcam's Caspase 6 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VEID-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 6 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 6 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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Notes
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests 2X Reaction Buffer NM 1 x 10ml Active Caspase-6 Purple 1 x 100 units Caspase Inhibitor, Z-VAD-FMK Brown 1 x 10µl Caspase Substrate VEID-AFC Brown 1 x 0.5ml DTT Green 1 x 100µl -
Research areas
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Relevance
Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei. -
Cellular localization
Cytoplasmic -
Alternative names
- Apoptotic Protease MCH2
- CASP6
- Caspase 6 apoptosis related cysteine protease
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