Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB6 antibody [21C11BC11] (ab110244)

Key features and details

  • Mouse monoclonal [21C11BC11] to NDUFB6
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-NDUFB6 antibody [21C11BC11]
    See all NDUFB6 primary antibodies

  • Description

    Mouse monoclonal [21C11BC11] to NDUFB6

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Western blot - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    All lanes : Anti-NDUFB6 antibody [21C11BC11] (ab110244) at 0.5 µg/ml

    Lane 1 : isolated mitochondria from Human heart at 5 µg
    Lane 2 : isolated mitochondria from cow heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 15 kDa

  • Flow Cytometry - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Flow Cytometry - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Overlay histogram showing HepG2 cells stained with ab110244 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110244, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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