Caspase-6 (active) Staining Kit - Green Fluorescence (ab219936)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Caspase-6 (active) Staining Kit - Green Fluorescence
See all Caspase-6 kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936) is a sensitive fluorometric assay to measure caspase 6 activation in live cells. The assay uses FAM-VEID-FMK, which binds irreversibly to active caspase 6 in apoptotic cells. The fluorescent intensity of the FAM-VEID-FMK signal is proportional to the amount of active caspase 6 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.
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Notes
Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Overexpression of caspase 6 promotes programmed cell death. It has been proven that caspase 6 has substrate selectivity for the peptide sequence Val-Glu-Ile-Asp (VEID). This kit uses FAM-VEID-FMK as a fluorescent indicator for caspase 6 activity. FAM-VEID-FMK irreversibly binds to activated caspase 6 in apoptotic cells.
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Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests 500X Hoechst 1 vial 500X Propidium Iodide 1 vial FAM-VEID-FMK 1 vial Washing Buffer 1 x 100ml -
Research areas
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Relevance
Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei. -
Cellular localization
Cytoplasmic -
Alternative names
- Apoptotic Protease MCH2
- CASP6
- Caspase 6 apoptosis related cysteine protease
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Images
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Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936)
Detection of active Caspase 6 in Jurkat cells. Jurkat cells (3 x 105 cells/100 μL/well) were either untreated (control) or treated with 1 μM staurosporine for 3 hours. Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. The fluorescent signal was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader (Molecular Devices) using bottom read mode
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Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936)Active caspase 6 staining in Jurkat cells. cells (3 x 105 cells/100 μL/well) were either untreated (A) or treated with 1 μM staurosporine for 3 hours (B). Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. Increase in fluorescent intensity was observed using a fluorescence microscope with a FITC channel.