Biotin Anti-Integrin alpha 5 antibody [5H10-27] (ab25189)
![Biotin Anti-Integrin alpha 5 antibody [5H10-27] (ab25189)](https://www.abcam.com/ps/products/25/ab25189/Images/ab25189-258669-anti-integrin-alpha-5-antibody-5h10-27-biotin-flow-cytometry.jpg "Biotin Anti-Integrin alpha 5 antibody [5H10-27] (ab25189)")
Key features and details
- Biotin Rat monoclonal [5H10-27] to Integrin alpha 5
- Suitable for: Flow Cyt
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Integrin alpha 5 antibody [5H10-27]
See all Integrin alpha 5 primary antibodies -
Description
Biotin Rat monoclonal [5H10-27] to Integrin alpha 5 -
Host species
Rat -
Conjugation
Biotin -
Specificity
ab25189 recognises alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor. -
Tested applications
Suitable for: Flow Cytmore details -
Immunogen
Tissue/ cell preparation of C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
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General notes
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody. -
Clonality
Monoclonal -
Clone number
5H10-27 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Biotin Anti-Integrin alpha 5 antibody [5H10-27] (ab25189)
Flow cytometry analysis staining Integrin alpha 5 in cells using ab25189 at a dilution of 1 μg/10⁶ cells.
