Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)
![Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)](https://www.abcam.com/ps/products/275/ab275977/Images/ab275977-411993-anti-alpha5beta1Integrin-antibody-ICCIF.jpg "Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [M200 (Volociximab)] to Integrin alpha 5 + beta 1 - Chimeric
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric -
Description
Rabbit monoclonal [M200 (Volociximab)] to Integrin alpha 5 + beta 1 - Chimeric -
Host species
Rabbit -
Specificity
The antibody binds to human α5β1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Fusion protein corresponding to Human Integrin alpha 5 + beta 1. Heterodimeric α5β1Fc fusion protein
Database link: P08648 -
Positive control
- ICC: HeLa cells. Flow cyt: PBMC cells.
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General notes
his chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M200 (Volociximab) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] (ab275977)
Immunofluorescence analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells permeabilized with 0.15% Triton and labeled Integrin alpha 5 + beta 1 with ab275977 at 10 µg/ml for 1h followed by Alexa Fluor®488 secondary antibody (1 µg/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab275977, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor®488 secondary antibody.
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![Flow Cytometry - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)](https://www.abcam.com/ps/products/275/ab275977/Images/ab275977-1-anti-alpha5beta1Integrin-antibody-ELISA.jpg)
Flow Cytometry - Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or ab275977 (blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.
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![Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)](https://www.abcam.com/ps/products/275/ab275977/Images/ab275977-3-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Chimeric (ab275977)