Anti-Integrin alpha 5 antibody (ab112183)
Key features and details
- Rabbit polyclonal to Integrin alpha 5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin alpha 5 antibody
See all Integrin alpha 5 primary antibodies -
Description
Rabbit polyclonal to Integrin alpha 5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: This antibody gave a positive signal in Human Placenta tissue lysate, JEG-3 whole cell lysate, HeLa whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications CowPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 114 kDa).IHC-P Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 114 kDa).IHC-P
Use a concentration of 1 µg/ml.Target
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Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3678 Human
- Omim: 135620 Human
- SwissProt: P08648 Human
- Unigene: 505654 Human
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Alternative names
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Images
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Western blot - Anti-Integrin alpha 5 antibody (ab112183)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Integrin alpha 5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jeg3 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab112183 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab112183 was shown to recognize Integrin alpha 5 when Integrin alpha 5 knockout samples were used, along with additional cross-reactive bands. Wild-type and Integrin alpha 5 knockout samples were subjected to SDS-PAGE. Ab112183 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (ab112183)IHC image of Integrin alpha 5 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112183, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Integrin alpha 5 antibody (ab112183)All lanes : Anti-Integrin alpha 5 antibody (ab112183) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Integrin alpha 5 contains an extensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References (1)
ab112183 has been referenced in 1 publication.
- Rattila S et al. Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin ?5ß1 Is a Modulator of Extravillous Trophoblast Functions. Cells 8:N/A (2019). PubMed: 31683744
Images
-
Western blot - Anti-Integrin alpha 5 antibody (ab112183)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Integrin alpha 5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jeg3 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab112183 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab112183 was shown to recognize Integrin alpha 5 when Integrin alpha 5 knockout samples were used, along with additional cross-reactive bands. Wild-type and Integrin alpha 5 knockout samples were subjected to SDS-PAGE. Ab112183 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (ab112183)
IHC image of Integrin alpha 5 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112183, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Western blot - Anti-Integrin alpha 5 antibody (ab112183)All lanes : Anti-Integrin alpha 5 antibody (ab112183) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Integrin alpha 5 contains an extensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
