Anti-Integrin alpha 5 antibody [P1D6] (ab78614)
![Anti-Integrin alpha 5 antibody [P1D6] (ab78614)](https://www.abcam.com/ps/products/78/ab78614/Images/Integrin-alpha-5-Primary-antibodies-ab78614-1.jpg "Anti-Integrin alpha 5 antibody [P1D6] (ab78614)")
Key features and details
- Mouse monoclonal [P1D6] to Integrin alpha 5
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-Integrin alpha 5 antibody [P1D6]
See all Integrin alpha 5 primary antibodies -
Description
Mouse monoclonal [P1D6] to Integrin alpha 5 -
Host species
Mouse -
Specificity
ab78614 is not reactive with the denatured Integrin alpha 5 subunit. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Integrin alpha 5.
Database link: P08648 -
Positive control
- Flow Cyt: HeLa cells, HT-1080 cells
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General notes
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
P1D6 -
Isotype
IgG3 -
Research areas
Images
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Flow Cytometry - Anti-Integrin alpha 5 antibody [P1D6] (ab78614)
Overlay histogram showing HeLa cells stained with ab78614 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine (ab120050) to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78614, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Integrin alpha 5 antibody [P1D6] (ab78614)](https://www.abcam.com/ps/products/78/ab78614/Images/ab78614-218450-ab78614.jpg)
Flow Cytometry - Anti-Integrin alpha 5 antibody [P1D6] (ab78614)Flow Cytometry Analysis: Representative lot data.
Cell surface staining of HT-1080 cells using 1 µg/mL of ab78614 (shaded histogram) and an isotype control (unshaded histogram). Cells were detected using a mouse IgG-FITC.This image was generated using the ascites version of the product.
