Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAM15 antibody [EPR5619] (ab124698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5619] to ADAM15
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-ADAM15 antibody [EPR5619]
    See all ADAM15 primary antibodies

  • Description

    Rabbit monoclonal [EPR5619] to ADAM15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ADAM15 aa 250-350 (extracellular). The exact sequence is proprietary.
    Database link: Q13444

  • Positive control

    • SW480 cell lysate; Human colon tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified ab124698 at 1:800 dilution (2.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution (purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 93 kDa
    Observed band size: 110,90 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution + SW480 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP

    Predicted band size: 93 kDa



    ab124698 also appears to recognize the pro-protein (110kDa). This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)

    ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.

  • Anti-ADAM15 antibody [EPR5619] (ab124698)
    Anti-ADAM15 antibody [EPR5619] (ab124698)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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