Biotin Anti-IL-1 alpha antibody (ab271258)
Key features and details
- Biotin Rabbit polyclonal to IL-1 alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies -
Description
Biotin Rabbit polyclonal to IL-1 alpha -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA MouseWB Mouse
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Immunogen
Recombinant fragment corresponding to Mouse IL-1 alpha aa 100 to the C-terminus.
Database link: P01582
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-IL-1 alpha antibody (ab271258)All lanes : Biotin Anti-IL-1 alpha antibody (ab271258) at 0.2 µg/ml
Lane 1 : Recombinant Mouse IL-1 alpha protein at 0.25 µg
Lane 2 : Recombinant Mouse IL-1 alpha protein at 0.125 µg
Lane 3 : Recombinant Mouse IL-1 alpha protein at 0.0625 µg
Lane 4 : Recombinant Mouse IL-1 alpha protein at 0.03125 µg
Lane 5 : Recombinant Mouse IL-1 alpha protein at 0.015625 µg
Lane 6 : Recombinant Mouse IL-1 alpha protein at 0.0078 µg
Lane 7 : Recombinant Mouse IL-1 alpha protein at 0.0039 µg
Lane 8 : Recombinant Mouse IL-1 alpha protein at 0.00195 µg
Lane 9 : Recombinant Mouse IL-1 alpha protein at 0.000975 µg
Lane 10 : Recombinant Mouse IL-1 alpha protein at 0.0004875 µg
Lane 11 : Recombinant Mouse IL-1 alpha protein at 0.00024 µg
Predicted band size: 31 kDa
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Sandwich ELISA - Biotin Anti-IL-1 alpha antibody (ab271258)Mouse IL-1 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271258. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse IL-1 alpha protein.