Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDC45L antibody [EPR5759] (ab126762)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5759] to CDC45L
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CDC45L antibody [EPR5759]
    See all CDC45L primary antibodies

  • Description

    Rabbit monoclonal [EPR5759] to CDC45L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CDC45L aa 500-600. The exact sequence is proprietary.

  • Positive control

    • 293T, Jurkat, and K562 cell lysates; Human colon tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] (ab126762)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] (ab126762)

    ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-CDC45L antibody [EPR5759] (ab126762)
    Western blot - Anti-CDC45L antibody [EPR5759] (ab126762)
    All lanes : Anti-CDC45L antibody [EPR5759] (ab126762) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] (ab126762)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] (ab126762)

    ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CDC45L antibody [EPR5759] (ab126762)
    OI-RD Scanning - Anti-CDC45L antibody [EPR5759] (ab126762)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CDC45L antibody [EPR5759] (ab126762)
    Anti-CDC45L antibody [EPR5759] (ab126762)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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