Anti-CDC45L antibody (ab230178)
Key features and details
- Rabbit polyclonal to CDC45L
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDC45L antibody
See all CDC45L primary antibodies -
Description
Rabbit polyclonal to CDC45L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CDC45L aa 1-270.
Database link: O75419 -
Positive control
- WB: K562, HEK-293T, HeLa and HepG2 whole cell lysate. IHC-P: Human kidney tissue. IP: K562 whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDC45L antibody (ab230178)All lanes : Anti-CDC45L antibody (ab230178) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 66, 60, 69 kDa
Observed band size: 58,66 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody (ab230178)Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-CDC45L antibody (ab230178)CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.
Lane 1: Control IgG IP in K562 whole cell lysate.
Lane 2: ab230178 in K562 whole cell lysate.
Lane 3: CDC45L in K562 whole cell lysate (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).
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Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody (ab230178)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.
Secondary is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
