Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5759] to CDC45L - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDC45L antibody [EPR5759] - BSA and Azide free
    See all CDC45L primary antibodies

  • Description

    Rabbit monoclonal [EPR5759] to CDC45L - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240032 is the carrier-free version of ab126762. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240032 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)

    ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126762).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)

    ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126762).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
    OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126762).

  • Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
    Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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