Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
![Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-264172-anti-vitamin-d-binding-protein-antibody-ep2923y-western-blot.jpg "Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-Vitamin D Binding protein antibody [EP2923Y]
See all Vitamin D Binding protein primary antibodies -
Description
Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.
-
Positive control
- Human liver tissue. Human plasma.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2923Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and Diluting buffer 5% NFDM /TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-96520-anti-vitamin-d-binding-protein-antibody-ep2923y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
![Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-4-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
