Anti-Vitamin D Binding protein antibody [19E8] (ab23485)
![Anti-Vitamin D Binding protein antibody [19E8] (ab23485)](https://www.abcam.com/ps/products/23/ab23485/Images/Vitamin-D-Binding-protein-Primary-antibodies-ab23485-1.jpg "Anti-Vitamin D Binding protein antibody [19E8] (ab23485)")
Key features and details
- Mouse monoclonal [19E8] to Vitamin D Binding protein
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-Vitamin D Binding protein antibody [19E8]
See all Vitamin D Binding protein primary antibodies -
Description
Mouse monoclonal [19E8] to Vitamin D Binding protein -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Vitamin D Binding Protein isolated from human plasma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
19E8 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)IHC image of ab23485 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
