Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3 - BSA and Azide free
  • Suitable for: Competitive ELISA
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free
    See all Vitamin D2+Vitamin D3 primary antibodies

  • Description

    Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Competitive ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Vitamin D2+Vitamin D3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Competitive ELISA: 25-OH-Vitamin-D.

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)
    Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)

    Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking. 
    This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)
    Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)

    ab229822 was used at 11.8 ng/ml.

    The results show cross-reactivity with Vitamin D2.
    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)
    Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (ab237700)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.