All lanes : Anti-Vitamin D Binding protein antibody [5E8] (ab23484) at 5 µg/ml

Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.