Anti-DNA Polymerase iota antibody (ab264193)
Key features and details
- Rabbit polyclonal to DNA Polymerase iota
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Polymerase iota antibody
See all DNA Polymerase iota primary antibodies -
Description
Rabbit polyclonal to DNA Polymerase iota -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide aa 690-715. The exact sequence is proprietary.
Database link: Q9UNA4 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DNA Polymerase iota antibody (ab264193) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds4-8% SDS-PAGE.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
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DNA Polymerase iota was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264193 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264193 at 1 µg/ml.
Lane 1: ab264193 IP in HeLa whole cell lysate.
Lane 2: Control IgG.