Anti-4-1BBL antibody [EPR1172Y] (ab68185)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1172Y] to 4-1BBL
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-4-1BBL antibody [EPR1172Y]
See all 4-1BBL primary antibodies -
Description
Rabbit monoclonal [EPR1172Y] to 4-1BBL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human 4-1BBL (C terminal). The exact sequence is proprietary.
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Positive control
- WB: 293T and Raji cell lysates. IP: HEK293 whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1172Y -
Isotype
IgG -
Research areas
Images
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Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25,32 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25-32 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.
Lane 1 (input): HEK293 whole cell lysate (10µg)
Lane 2 (+): ab68185 + HEK293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
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