Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15135] to Cathepsin W - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free
    See all Cathepsin W primary antibodies

  • Description

    Rabbit monoclonal [EPR15135] to Cathepsin W - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251056 is the carrier-free version of ab191083. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251056 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    All lanes : Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/50000 dilution

    Lane 1 : HuT-78 cell lysate
    Lane 2 : MOLT-4 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab191083, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    This data was developed using ab191083, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/10000 dilution + PC12 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab191083, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    All lanes : Anti-Cathepsin W antibody [EPR15135] (ab191083) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab191083, the same antibody clone in a different buffer formulation.

     

  • Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)
    Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (ab251056)

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