Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19846] to Uteroglobin - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free
    See all Uteroglobin primary antibodies

  • Description

    Rabbit monoclonal [EPR19846] to Uteroglobin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat lung tissue.

  • General notes

    Ab232562 is the carrier-free version of ab213203. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232562 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19846

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

    Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

    The nuclear counterstain is DAPI (blue).

    Heat mediated antigen retrieval using citrate buffer pH 6.0

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on the airway epithelium of mouse lung [PMID: 22916196].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on the airway epithelium of rat lung [PMID: 22916196].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung lysate 10ug (Input).

    Lane 2: ab213203 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.  Strong cytoplasmic staining on the airway epithelium of rat lung is observed [PMID: 22916196]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

  • Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)
    Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (ab232562)

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